Identification of a number of important EC-specific or EC-highly expressed molecules, such as plateletĮndothelial cell adhesion molecule (PEC AM- 1 or CD31), vascular endothelial cell adhesion Over the past several decades, massive efforts towards this direction have resulted in the Interest in identifying new genes specifically or preferentially expressed in vascular endothelial cellsĪnd studying their biological functions, which will help to develop more specific and effective pro- and EndothelialĬells (ECs) constitute the inner layer of blood vessels and are the important players in angiogenesis,Īnd EC migration is a key step during angiogenic process. Rheumatoid arthritis, diabetic retinopathies, and age-related macular degeneration. Wound healing, and female reproductive functions, but also critically involved in many pathologicalĬonditions, such as ischemic vascular diseases, atherosclerosis, tumor growth and metastasis, It is not only fundamental for normal organ growth and development, Keyword: ECSCR/ECSM2 alternative splicing isoform gene structure exon-intronīoundary cDNA cloning and expression endothelial cell migrationĪngiogenesis or neovascularization is a physiological process by which new blood vessels developįrom pre-existing vasculature. Understanding of alternative splicing of ECSCR. We have also defined the gene structure of mouseĮCSCR using bioinformatics tools, which provides new information towards a better They are differentially expressed in a variety of tissue types and likely involved in Taken together, we have provided several Unes ofĮxperimental evidence that two mouse ECSCR splicing variants/isoform precursors exist. Our results showed that both isoforms significantly inhibited vascular epidermal growthįactor (VEGF)-induced cell migration. MSI cells overexpressing GFP alone, isoform-1 -GFP, and isoform-2-GFP, respectively. Finally, we performed cell migration assays using mouse endothelial Fluorescence microscopy revealed thatīoth ECSCR isoforms are localized at the cell surface, which is consistent with the Suggesting that both isoforms are glycoproteins. Interestingly, the actual sizes of either ECSCR-GFP or -FLAGįusion proteins detected by immunoblotting are much larger than their predicted sizes, We expressed GFP- and FLAG-tagged ECSCR isoforms, respectively, in an ECSCRĭeficient cell line (HEK293). To further explore their potential cellular functions, ![]() Isofonn-2 is the predominant form, which was most abundant in heart, lung, and muscles,Īnd moderately abundant in uterus and testis. Quantitative RT-PCR results revealed that Suggested that they are alternative splicing variants (ECSCR isoform- 1 and -2), differingįrom each other in the first and second exons. Nucleotide sequence and exon-intron junction analyses RT-PCR, which encode two putative ECSCR isoform precursors with considerable In the present study, we cloned two mouse full-length cDNAs by Human endothelial cell-specific molecule 2 (ECSM2)/endothelial cell-specific chemotaxis We have previously reported molecular characterization of Vascular ECs and in-depth understanding of their biological functions may lead to discovery ![]() Thus, identification of genes that are specifically or preferentially expressed in Players in blood vessel formation, and EC migration is a key component of the angiogenic Received: 8 February 2012 in revised form: 19 March 2012 /Accepted: 20 March 2012 /Ībstract: Endothelial cells (ECs) that line the lumen of blood vessels are important * Author to whom correspondence should be addressed E-Mail: Tel.: -1-1-60 Fax: -1-1-60. USA E-Mail: Current address: Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Technology, Wuhan, Hubei 430030, China E-Mail: of Biochemistry, University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA E-Mail: of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Joseph's Hospital and Medical Center, Phoenix, AZ 85013, Joseph's Hospital and Medical Center, Phoenix,ĪZ 85004, USA E-Mails: (C.S.) (P.M.) (J.B.)īarrow Neurological Institute, St. ^ Department of Obstetrics and Gynecology, St. Wen Wu ^'^'^ Chunwei Shi \ Fanxin Ma \ James Balducci \ Hanju Huang ^ Hong-Long Ji ^ Splicing Variants of Mouse Endothelial Cell-Specific Structural and Functional Characterization of Two Alternative Full text of " Structural and Functional Characterization of Two Alternative Splicing Variants of Mouse Endothelial Cell-Specific Chemotaxis Regulator (ECSCR)."
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